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KMID : 0603820090150040273
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.273 ~ p.279
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The Inhibitory Effects of Cordycepin (3’-deoxyadenosine) on Thapsigargin-enhanced Cytosolic Ca2+-influx and -mobilization in Human Platelets
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Cho Hyun-Jeong
Park Hwa-Jin
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Abstract
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Cordycepin (3’-deoxyadenosine) is an adenosine analogue isolated from Cordyceps militaris, and it has been used as an anti-cancer and anti-inflammation ingredient in traditional Chinese medicine. We investigated the effects of cordycepin on human platelet aggregation induced by thapsigargin, and determined the cytosolic free Ca²? levels ([Ca²?]i), an aggregation-stimulating factor. Cordycepin significantly inhibited thapsigargin-induced platelet aggregation. Its inhibitory effect was continually sustained at the maximal aggregation concentration of thapsigargin. The thapsigargin-induced [Ca²?]i were clearly reduced by cordycepin in the presence of exogenous CaCl₂ or extracellular Ca²?-chelator (EDTA). These results suggest that cordycepin inhibited thapsigargin-induced Ca²?-influx from extracellular domain and thapsigargin-induced Ca²?-mobilization from intracellular Ca²? storage. Accordingly, our data demonstrated that cordycepin may have a beneficial effect on platelet aggregation-mediated thrombotic diseases by inhibiting a [Ca²?]i-elevation.
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KEYWORD
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Cordycepin, Platelet aggregation, Intracellular Ca²?, Anti-platelet activity, Thapsigargin
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